![]() Finalize your acquisition and get publication-ready microscope images with TruSight image processing.Identify regions of interest on the Overlay Map, then switch to a higher magnification objective for high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR).Use a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of whole specimens.The FV3000 microscope’s macro-to-micro workflow provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging. Macro-to-Micro Imaging and Super Resolution Microscopy Maximum intensity projection in Z with TruSight deconvolution processing. Images were acquired using a UPLXAPO10X objective with 405 nm, 488 nm, 561 nm, and 730 nm laser lines on GaAsP and GaAs detectors. (b) Rat brain slice labeled with Hoechst (blue), anti-IBA1 (Alexa Fluor 488 green), anti-MAP2 (Alexa Fluor 594, yellow), anti-FOX3/NeuN (Alexa Fluor 647 red), and anti-MBP (Alexa Fluor 750 cyan). Price, Ph.D., Price Lab, Eugene McDermott Professor, Director, Center for Advanced Pain Studies, Department of Neurobiology, School of Behavioral and Brain Sciences, University of Texas at Dallas. Image data courtesy of Stephanie Shiers, Ph.D. (a) Mouse mPFC labeled with glial fibrillary acidic protein (GFAP astrocyte marker yellow), calmodulin-dependent protein kinase II (CaMKII pyramidal neuron marker red), amphoterin-induced protein 1 precursor (AMIGO-1 neuronal membrane marker cyan), parvalbumin (PV inhibitory neuron marker purple), ankyrin-G (AnkG axon initial segment marker green), and nuclear yellow (nuclei marker blue). X Line objectives offer the widest range of chromatic correction, from 400 nm – 1000 nm, for exceptional color accuracy during multicolor fluorescence imaging.TruFocus Red enables stable and gentle NIR time-lapse imaging.730 nm or 785 nm lasers and an NIR-sensitive 1- or 2-channel GaAs detectors enable up to 6 channels for multiplexed imaging from violet to NIR (400 nm–890 nm).Our new FV3000 Red near-infrared (NIR) solution further extends the FV3000 microscope’s wavelength detection capabilities to the NIR region of up to 890 nm through a suite of carefully engineered NIR upgrades: Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals.Variable barrier filter mode provides simultaneous four-channel image acquisition with up to sixteen channels in sequential mode. Offers independently adjustable channels to optimize signal detection for each individual fluorophore.traditional spectral detection technology by implementing the volume phase holographic (VPH) diffraction grating. TruSpectral technology achieves up to 3X more light transmission vs.Using proprietary spectral detection technology, the FV3000 confocal microscope's TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores. ![]() High-Sensitivity Image Multiplexing from Violet to NIR ![]() Featuring the high sensitivity and speed required for live cell imaging as well as deep tissue observation, the FV3000 confocal microscope enables a wide range of imaging modalities, including macro-to-micro imaging, super resolution microscopy, and quantitative data analysis. The FLUOVIEW FV3000 series of confocal laser scanning microscopes meets some of the most difficult challenges in modern science. Next Generation FLUOVIEW for the Next Revolutions in Science ![]()
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